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Ninety-seven - The Misunderstanding of Cloning

In the end, only 33 of the 50 elderly people met the experimental requirements after physical examination and bone age test.

The remaining seventeen that did not meet the requirements were naturally returned.

Fortunately, this Gorob Village was able to find so many elderly people over the age of 50. I heard that the village chief even went to the neighboring village to negotiate with dignitaries in order to gather enough people.

The reason why the elderly are scarce is because of the backward medical level. The towns are better, and there are not many people in the villages who can live to the age of 50 or more.

According to official statistics in Namibia, more than 10 percent of children in this country are orphans because their parents died of AIDS shortly after giving birth to them.

In Namibia, the national population is only 2.58 million, but the HIV infection rate has reached 21.3%, that is to say, one fifth of the people in this country are HIV carriers.

Fortunately, Namibia's HIV infection rate is not too high in Africa, far less than Botswana's 37.3%, Swaziland's 38.8%, and Lesotho's 28.9%...

Africa has two world-famous specialties, one is diamonds and the other is AIDS.

Ignoring the dissatisfaction of Gorob Village, Chen Chen directly asked the 33 people to take a bath, and then arranged for them to live in the basement and provide food and drink, but restricted their activity area.

At the same time, in the laboratory, Chen Chen recruited a group of experienced assistants and began to clone the cloned human embryos of these old men.

For the specific operation, Chen Chen used a more advanced three-dimensional polarized optical spindle imaging system. After locating the nucleus of the egg cell, he used a laser to punch holes in the zona pellucida, and removed the nucleus in a mode that did not damage the egg cell. Somatic cell nuclei of the elderly can be implanted with a micromanipulator.

But unfortunately, the technology of this group of people is completely incomparable to Chen Chen. They failed several times in the middle, and also failed several times in the process of using electric current and chemical induction. In the end, all the clones were completed under the guidance of Chen Chen. embryo.

Next, the cloned embryos take 14 days to grow to the blastocyst stage, and then the embryonic stem cells inside are extracted.

The reason why the international community is against cloning human embryos is actually against this step. For some people, killing these fertilized eggs is tantamount to killing people, which is ethically unacceptable.

Fortunately, these objections did not hinder the study of cloned embryos. So far, the technology of cloning embryonic stem cells in various countries is still making great strides forward.

After the embryonic stem cells are extracted, by controlling the growth environment of the embryonic stem cells and manipulating genetically specific gene expression, these embryonic stem cells can be differentiated into the desired hematopoietic stem cells and mesenchymal stem cells.

The stem cells cloned by this method have almost the same genes as the old man, there will be no rejection reaction, and they are vigorous stem cells.

After these elderly people have undergone chemotherapy and radiotherapy, they will be implanted into the human body to replace the already aging stem cells in the elderly.

Once these young hematopoietic and mesenchymal stem cells return to the bone marrow, they will rapidly differentiate into blood cells, muscle cells, liver cells, osteoblasts, fat cells, chondrocytes, matrix and other human cells, repairing and improving the aging damage of the human body .

That's what stem cells do.

But for a while, the academic world has been caught in a huge misunderstanding, which was fermented by the earliest clone of Dolly the sheep—

As we all know, the cloned sheep Dolly is the earliest artificially cloned animal in the world. Before Dolly was born, its donor was a Finnish Dorset white-faced sheep.

Dolly was cloned by extracting mammary gland cells from the sheep and removing the nucleus from them.

In other words, Dolly's genes are basically the same as this Dorset white-faced sheep.

And this Finnish Dorset white-faced sheep was 6 years old when its mammary gland cells were extracted.

However, after the cloned sheep Dolly was born, it was found that Dolly's telomeres were shorter than those of sheep of the same age.

Not only that, Dolly the cloned sheep developed symptoms of premature aging at the age of 6. Although people took good care of it, Dolly eventually died.

In the same year, the cloned sheep Dolly's donor, the Dorset White-faced sheep, also died at the age of twelve.

In the biological research on lifespan, the more mainstream theories are "telomere theory" and "free radical theory", both of which have not been fully confirmed, but if telomeres are the key factor affecting lifespan, then cloned sheep Dolly's Death seems to have a basis.

Because the nucleus donor of the cloned sheep Dolly is a six-year-old sheep, although the cloned sheep Dolly is just born, the telomere length has actually been worn down to only six years of life? So it will die at the same time as the donor after 6 years?

At that time, this misunderstanding affected the entire academic world and cast a shadow over the entire cloning technology.

Because once this conclusion is true, it shows that most of the treatments developed by cloning technology are futile, no matter how cloning is done, the length of the telomere is as much as it is, and it will never change...

This misunderstanding even affected sci-fi works after 2000, causing many sci-fi works to set the short-lived defect of clones invariably when setting clones.

But fortunately, at the end of 2019, this misunderstanding was finally revealed——

In November 2019, Professor Li and his team of the Key Laboratory of Animal Reproduction Regulation and Breeding in Zhongzhou District made a decisive conclusion in the study of telomeres in cloned animals:

After years of research, Professor Li and his team found that telomere reprogramming occurs in cloned embryos during the activation of the zygotic genome. Regardless of the telomere length of the donor cell, the reprogramming process can be restored to the original length, that is, the donor. its own length!

Not only that, Professor Li also found that the length of telomeres is actually related to the development of cloned embryos.

During the cloning process, the telomeres of cloned embryos with normal development can be significantly restored, while the telomeres of cloned embryos with abnormal development will not be restored, but will be shortened.

In addition, Professor Li also found that adding some small molecular substances such as trichostatin A, sodium butyrate, melatonin, etc. during the culture of cloned embryos can effectively improve the telomere growth rate in a hypoxic environment. Reprogramming efficiency!

The research results were published in The FASEB Journal, the journal of the North American Federation of Experimental Biology Societies. The title of the paper is "Inhibiting repressive promotes telomere in somatic cell reprogramming".

So far, the misunderstanding that the lifespan of cloned animals is limited by the donor has been completely broken.

As for why the cloned sheep Dolly had premature aging, and why was the telomere length insufficient? Then the benevolent see benevolence and the wise see wisdom.

While the laboratory was cultivating the cloned embryos of these old men, Dlamini finally sent over the people on Chen Chen's list.

This time, Dlamini sent a total of 30 people, all of whom were the elite among the elites. I am afraid that the trump card of Cheng Chao would not be conspicuous.

As long as Chen Chen can subdue this group of jackal-like mercenaries, the foundation for establishing a quick-response force will be complete in the future.

Chen Chen's goal is to build a private armed force that can carry out precise global strikes in the next few years. The main purpose is to protect the company's properties around the world and destroy any evidence that is detrimental to the company.

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